There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.
(Oooh) Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.
Just mix your template with a buffer and some primers,
Nucleotides and polymerases too.
Denaturing, annealing, and extending,
Well it’s amazing what heating and cooling and heating will do.
[Chorus]
PCR when you need to detect mutation (detect mutation)
PCR when you need to recombine (recombine)
PCR when you need to find out who the daddy is (who’s your daddy?)
PCR when you need to solve a crime (solve a crime)
[x2]
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(PCR) To all the scientists out there doing PCR, Bio-Rad salutes you with the all-new 1000 series thermal cycling platform.
Randolph: Six years of graduate school, and look at our boy now.
Randolph’s Wife: Oh Randolph! Now I remember somebody who wasn’t too different from this not too long ago.
Enzyme, whatcha doin’ today?
I said enzyme, want to make DNA?
I said enzyme, show me your binding way
Let me mix you with some primers
Enzyme, time to build a new strand
I said enzyme, cause I know that you can
I said enzyme, these letters also spell DAN
All we need to get things started
Is just a little bit of GTCA
Is just a little bit of GTCA
I can build DNA
I can be a big star
You’re the key to my PCR
Thats right I’m talkin’ bout GTCA
Just a little bit of GTCA
I can make some new strands
I can view all my bands
I can be the best that I can
Enzyme, you have come a long way
But in my lab, demands are higher today
I said enzyme, now its time to go fast
We have got to increase throughput
Enzyme, I think I found what I need
It’s an enzyme, it gives me much higher speed
It goes so fast, when it builds DNA
All we need to get it started
Is just a little bit of GTCA
Is just a little bit of GTCA
I can build DNA
I can reach for the stars
I can do faster PCR
Yeeeeeaaaa-eeee-ehhh!
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